Although the former Ipswich Town star is already an Under-21 international coach Stuart Baxter has decided to name him in his squad for the tournament that takes place from July 16-26.
England face Austria, France and the Czech Republic in their group while the other group contains the hosts together with Italy, Portugal and Norway.
The news means that Ambrose looks certain to miss the club’s trip to Malaysia for the Asia Cup.
Full squad:
Justin Hoyte (Arsenal), Liam Ridgewell, Peter Whittingham (both Aston Villa), Ciaran Donnelly (Blackburn Rovers), Wayne Routledge (Crystal Palace), Lee Camp (Derby County), Steven Schumacher (Everton), Dean Leacock (Fulham), Tommy Wright (Leicester City), John Welsh (Liverpool), Lee Croft, Dorryl Proffitt (both Manchester City), David Jones, Luke Steele (both Manchester United), Andrew Davies, Stewart Downing (both Middlesbrough), Darren Ambrose (Newcastle United), Marcel McKie (Tottenham Hotspur).