Craig Levein's men suffered their first loss of the campaign when Roman Hubnik grabbed the only goal of the game in Prague.
Gary Caldwell: "We are still on course for finishing second.
"That was probably always the objective. Spain on Tuesday is a game we will go into and give everything.
"Everyone in the world wouldn't expect us to get anything and that's when Scotland are usually at their best. So we can have a right go on Tuesday night.
"I thought it (Levein's tactics) worked very well. They looked very frustrated, you could see that in their play, and I don't think they had many chances.
"In the second half, they started to get a few more efforts and Allan McGregor made some good saves.
"But it was working, it was 0-0 and a point would have been a great result. It was just disappointing to lose from a set-piece."